taq enzyme
- 网络Taq酶
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This article emphasizes particularly on the effect of Taq enzyme on the result of PCR quantitative detect .
本文重点探讨Taq酶对PCR定量检测结果的影响。
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During the action of PCR , Taq enzyme has an important effect on the result of PCR quantitative detect .
在PCR反应过程中,Taq酶对PCR定量检测结果起到很大的影响。
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Basic knowledge of analytical reagents & alkalis PCR reaction system , Taq enzyme , primer et al .
PCR反应体系、Taq酶、引物,Tris碱及聚丙烯酰胺等;
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The satisfactory Taq enzyme amount is 1U ;
以1U酶量效果最满意;
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A DDRT-PCR system was established by perfecting the conditions of Mg ~ ( 2 + ), annealing temperature , template concentration and Taq enzyme .
通过对退火温度、模板用量、Taq酶和Mg~(2+)浓度的优化,建立了适合多枝赖草基因差异显示的DDRT-PCR体系。
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Screening ISSR-PCR the template concentration , primer concentration , dNTP concentration and Taq enzyme dosage , optimizing ISSR-PCR system . 4 .
分别筛选ISSR-PCR扩增的模板浓度、Taq酶用量、引物浓度及dNTP浓度,优化ISSR-PCR扩增体系。
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Taq enzyme concentration 2U ;
Taq酶用量为2U;
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The multiplex amplification conditions such as the concentration of primer , Mg2 + , Taq enzyme , anneal temperature , cycle times , and the time of pre-denaturation , final extension were optimized .
调节各基因座引物浓度、Mg2+浓度、Taq酶浓度、退火温度、循环次数、预变性时间、延伸时间等条件对复合体系进行优化,得到最佳的反应条件。
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Results For LCN template DNA , if only increased the amount of Taq enzyme or the reaction volume , the results of STR amplification were scarcely improved , but if increased PCR cycles , the detection sensitivity were significantly improved .
结果对低拷贝模板DNA,单纯增加Taq酶量或反应体系,扩增效率改善不明显;增加循环数,显著提高检验灵敏度;
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The result indicates that the ( amplification ) capability of Tgo enzyme is the same as that of Taq enzyme . But the amplification fidelity of Tgo enzyme is over 30 times higher than that of Taq enzyme and 1.6 times higher than that of Pfu .
该酶的扩增性能与Taq酶相当,而扩增忠实性分别比Taq酶、Pfu酶高30倍和1.6倍。
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These factors included : the concentrations of Taq polymerase enzyme , MgCl 2 , template , dNTPs , primer , amplification of paired primers and thermal cycles .
这些因素包括:Taq酶、MgCl2、模板、dNTPs、引物浓度,双引物扩增及热循环数。
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The results show that after of drug action , Taq estriction enzyme eLectrophoresis changed significantly . Show that Drugs and DNA binding sites o may be in two enzymes restriction site T / CGA .
结果表明DNA与药物作用后,Taq酶切电泳图发生明显改变,说明药物与DNA的结合位点可能在两种酶的酶切位点T/CGA上。
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The amplification capability , the heat tolerance and the ability to long PCR of Tgo DNA polymerase were examined . The results reveal that Tgo DNA polymerase has the same amplification function as Taq ( enzyme ) but with a high fidelity .
检测了重组Tgo酶的扩增性能、耐热性以及长距离PCR能力,结果表明,该重组酶扩增性能与Taq酶相当;